Team:Hong Kong-CUHK/DOC NBK JUL

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NOTEBOOK - JULY

1. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.


2. Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing.
3. Make plate with 0.5% soft agar with antibiotics- chloramphenicol.
4. Make plate with antibiotics –Ampiclin.
5. Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.


6. Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate.
7. Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells.
8. Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect.


9. Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.
10. Make LB agar solution


11. Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
12. No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells.


13. Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.
14. Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
15. Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.
16. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.


17. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing.
18. Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct.
19. The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics.


20. Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar     plate for testing using normal light bulb with its corresponding antibiotics.
21. Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.
22. Plasmids containing HsSRI-HsHtrI-Ectsr -backbone gene were sent to sequencing.
23. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.

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